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Click the Tools button (looks like a little briefcase) in the upper right of the dialog box to access a menu that includes commands for accessing the Bridge, creating a new project, editing file properties, creating new folders, and looking at or restoring deleted files. This menu also offers a command for going back to the OS Dialog (OS standing for Operating System), which you also can do by clicking the Use OS Dialog button that has replaced the Use Adobe Dialog button in the lower-left corner of the dialog box. There also is an Adobe dialog version of the Save and Save As dialog boxes look for coverage of these later in this chapter.

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receptor The database was used to construct a phage cocktail consisting of 37 different phages The 37-phage cocktail was screened against 58 E coli O157 : H7 isolates, by placing a drop of phage lysate (1011 PFU/ ml) on a lawn of each individual bacterial isolate After overnight incubation, the zone of clearing was checked for the presence of bacterial colonies, which would indicate bacterial mutants that were resistant to the cocktail The cocktail produced complete clearing on all of the isolates, and no resistant colonies were observed In addition, the phage cocktail lysate was dropped on an agar plate containing a lawn of a mixture of all 58 of the E coli O157 : H7 isolates After overnight incubation, no colonies were observed within the zone of clearing, indicating the absence of phage-resistant cells The cocktail was examined for its ability to reduce the presence of E.

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coli O157 : H7 in bovine fecal slurries and in calves In an anaerobic in vitro model, the phage cocktail completely eliminated a strain mixture of 104 cfu/ml of E coli O157 : H7 from bovine fecal slurries, within 4 h To evaluate the phage cocktail in live animals, a total of 14 Black Angus calves ranging from 4 to 6 months of age were orally inoculated with 108 cfu E coli O157 : H7 The in vivo experiments consisted of two trials The rst trial evaluated ileal samples and the second trial evaluated fecal samples for the presence of E coli O157 : H7 and phages In the rst trial, a signi cant decline in the concentration of E coli O157 : H7 in the ileal samples was observed at 8 h (P = 005) However, the concentration of E.

coli O157 : H7 increased back to the concentration of the control samples at 16 h In the second trial, shedding of E coli O157 : H7 decreased signi cantly in the treated group (P = 005) at 24 h Similar to the ileal samples, an increase in the concentration of E coli O157 : H7 was observed at 36 h in the fecal samples The increases in cell concentration were associated with a decrease in phage concentration None of the E coli O157 : H7 cultured from ileal or fecal samples showed resistance to the phage cocktail These results highlight the ability of the 37-phage cocktail to eliminate E coli O157 : H7 in fecal slurries, and to reduce the concentration of E coli O157 : H7 in calves, without the formation of phage-resistant mutants.

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